Transcriptional response of blood leukocytes from turkeys challenged with Salmonella enterica serovar Typhimurium UK1

• Turkeys were effectively colonized by Salmonella without detectable clinical signs. elicited immune-activating transcriptome responses in turkey leukocytes. impacted the STAT3 pathway, toll-like receptors, and cytokine signaling. IL1B was predicted as a major regulator of inflammatory gene expression. Non-typhoidal is one most common causes bacterial foodborne disease consumption contaminated poultry products, including turkey, source exposure. Minimizing colonization commercial turkeys could decrease incidence -associated human illness. Understanding host to these bacteria critical developing strategies minimize reduce food safety risk. In this study, we evaluated load blood leukocyte transcriptomic 3-week-old challenged with enterica serovar Typhimurium ( S . Typhimurium) UK1 strain. n = 8/dose) inoculated oral gavage 10 8 or colony forming units (CFU) UK1, fecal shedding tissue measured across multiple days post-inoculation (dpi). Fecal 1–2 log higher CFU group than group, but both doses crop, spleen, ileum, cecum, colon, bursa Fabricius cloaca causing any signs either birds. Blood leukocytes isolated from subset birds 3–4/dpi) pre-inoculation (0 dpi) 2 dpi their assayed RNA-sequencing (RNA-seq). At dpi, 647 genes had significant differential expression (DE), large increases immune such CCAH221 , IL4I1 LYZ IL13RA2 IL22RA2 ACOD1 IL1? DE leukocytes, which activate cell migration, phagocytosis proliferation, impact receptor pathways. These analyses revealed pathways responded pathogen can provide potential targets for intervention diagnostic assays mitigate turkeys.